《植物生理学报》 2015, 51(1): 73-78

拟南芥核酸外切体亚基RRP41L与RRP42及RRP45B的互作关系

杨敏¹, 王翠玲¹, 韩玉珍^2,*

¹河南科技大学农学院, 河南洛阳471003; ²中国农业大学生物学院, 植物生理生化国家重点实验室, 北京100193

通信作者：韩玉珍；E-mail: hanyuzhen@cau.edu.cn；Tel: 010-62733807

摘　要：

在真核生物中, 核酸外切体(exosome)是一个由9个核心蛋白亚基组成的在进化上十分保守的大分子复合物, 具有加工和降解RNA的重要功能。为了了解影响拟南芥生长发育的核酸外切体亚基RRP41-LIKE (RRP41L)与其他外切体亚基之间的互作关系, 本文利用酵母双杂交和荧光素酶互补技术从体外和体内两个方面验证了RRP41L与另外两个核酸外切体亚基RRP42及RRP45B的互作关系。结果显示RRP41L不能与RRP42互作, 而能与RRP45B互作。

关键词：拟南芥; RRP41L; 酵母双杂交; 荧光素酶互补技术

收稿：2014-09-19   修定：2014-11-26

资助：国家自然科学基金(30670192)、博士科研启动基金(09001770)和校青年基金(2014QN057)。

Interaction Relationship between RRP41L and RRP42, RRP45B, Three Subunits of Exosome in Arabidopsis thaliana

YANG Min¹, WANG Cui-Ling¹, HAN Yu-Zhen^2,*

¹College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471003, China; ²State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences,China Agricultural University, Beijing 100193, China

Corresponding author: HAN Yu-Zhen; E-mail: hanyuzhen@cau.edu.cn; Tel: 010-62733807

Abstract:

In eukaryotic cells, the exosome is an evolutionarily conserved macromolecular complex with nine subunits, mediating numerous reactions of 3'→5' RNA processing and degradation. To get around the interaction relationship between the RRP41-LIKE (RRP41L) and other subunits of exosome in Arabidopsis thaliana, yeast two-hybrid assay and firefly luciferase complementation imaging assay (LCI) were used to confirm the interaction relationship between RRP41L and other two core subunits of exosome, RRP42 and RRP45B in vitro and in vivo. Our results showed that RRP41L interacted with the RRP45B, but not interacted with the RRP42.

Key words: Arabidopsis thaliana; RRP41L; yeast two-hybrid assay; LCI